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As discussed in 'Materials and methods', such conditions are ideal to minimize cell heterogeneity and to obtain high quality equilibrium networks that are ideally suited for theoretical analysis.
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To minimize cell attachment, plates were precoated with 1 % agarose.
During this incubation, the cells were widely dispersed to minimize cell-cell contact.
This sampling on plates was preferred over inoculation with droplets, to minimize heterogeneity among cells.
We have used primary tendon cells (up to passage 3) isolated from three young male healthy donors (T1, T2, and T3) of similar age (27 ± 1.4 years), in order to minimize heterogeneity of cells among donors.
Secondly, the samples should not display apparent tumor cell heterogeneity.
They propose that heterogeneity in vascular density and blood flow are critical factors promoting cell heterogeneity.
Ideally, in order to minimize heterogeneity between different cell populations, a small aliquot of cells from each time point used for immunoblotting should be used for immunofluorescence analysis.
Furthermore, isolation of Bergmann glia from a restricted region of the cerebellum should minimize developmental heterogeneity between individual cells.
Accordingly, production cell lines are 'cloned', or derived from a single cell, in order to minimize heterogeneity (International Conference on Harmonisation (ICH), Guideline Q5D, 1997).
In order to minimize this heterogeneity and to achieve more consistent performance we used an approach that was previously used for cell-based toxicity assay development, namely, selecting the highest producing clone.
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