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After determining the center of the receptive field, light circles (6 12° in diameter) were presented for 300 ms. An inter-stimulus interval (duration, 5 s) was introduced to minimize cell adaptation.
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To minimize cell attachment, plates were precoated with 1 % agarose.
During this incubation, the cells were widely dispersed to minimize cell-cell contact.
Spectral sensitivity data were corrected for equal energy and a 0.5-mV response criterion was used to minimize light adaptation of dark-adapted retinas.
All collected strains were frozen within three generations of collection to minimize laboratory adaptation.
Through early detection, ED-SSS may help minimize the adaptation of avian influenza virus to human populations.
In the SF medium screening experiments, the direct adaptation method for cell growth is considered to be the easiest way to minimize adaptation time switching from SC medium to SF medium.
Cell clumping was minimized by stepwise adaptation to suspension culture: removing cell aggregates at intervals.
To minimize effects of adaptation, the inter-stimulus presentation interval was 30 s.
To minimize effects of adaptation (Wang et al., 2010) we considered only touches that occurred after long inter-touch intervals (>250 ms).
The rinsing time was chosen to minimize cross-adaptation between stimuli.
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