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When plants were eight weeks old we sampled young leaves from each line directly into liquid nitrogen to minimize breakdown of glucosinolates.
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Moreover, a further precaution against a loss of the already low peptide levels in microdialysis samples was the addition of the peptidase inhibitor bacitracin to the perfusion fluid to minimize enzymatic breakdown.
Stocks consisting of triple knockouts and Don Juan GFP were constructed over apterous Xa, a reciprocal translocation between the second and third chromosomes, which placed the second chromosome CG9000 region on the third chromosome, minimizing recombinatorial breakdown.
To minimize disturbances and breakdowns, e-maintenance and process supervisory control systems are being developed and introduced.
This might be combined with cell lysis procedures, either chemically or by the use of an autolytic vector, to minimize biases by enzymatic breakdown of the gene product, toxicity or secretion problems.
But we try to minimize any type of breakdowns we have using all the policies and procedures we have in place".
(If it's a go, you should also recommend precautions to minimize risk, and provide a breakdown of costs and potential benefits).
Finite element electrostatic modeling software is used to evaluate the electric field distribution around the sensor components, and improvements are incorporated at the design stage to minimize the possibility of electrical breakdown in the prototype device.
With the application of a diffraction limited wave front it was possible to minimize one factor during optical breakdown generation which contributes to a raise in threshold energy.
Risk-based maintenance (RBM) approach helps in designing an alternative strategy to minimize the risk resulting from breakdowns or failures.
Mohideen et al. (2011) presents a proposal to minimize the recovery time and the breakdown cost in the system in a construction plant (McNaught and Chan 2011).
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