Sentence examples for minimize amplification of from inspiring English sources

The following primer sets were designed to selectively amplify HCN1-4 mRNandnd span introns to minimize amplification of genomic DNA: HCN1- TCAAGGAGGCAGTATCAAGAGAAG and ACCGAAAGGGAGTAAAGACGAC; HCN2 – ACTGCCCGCTGACTTCC and ATCTCCTTGTTGCCCTTGGT; HCN3– CACCGCCCTCATCCAGTC and CCCTCACGCACCACCAG; and HCN4 – TTTCATCTCCTCCATCCCTGTC and CCTGCCGTCCATACCCAAT.

In order to minimize amplification of multiple loci, genomic regions with little or no homology to other regions were selected.

We therefore developed a method to minimize amplification of this gene before the cloning step intended to obtain SRK sequences.

PCR-primers were chosen to span at least one exon/intron boundary to minimize amplification of contaminating genomic DNA.

To minimize amplification of homologous genomic sequences, primer pairs were filtered using the UCSC In Silico PCR software, and only pairs yielding a single product were used.

High diversity among the potentially functional alleles led to the design of a degenerate primer pair, however attention was paid to minimize amplification of alleles with stop codons.

All primers were obtained (from Eurofins MWG, Ebersberg, Germany) and matched regions with an identical nucleic acid sequence in compared genomes and DNA sequences of the genus Francisella to minimize amplification failure of screened isolates.

To minimize amplification bias due to differential incorporation of fluorescent dyes, we opted for an indirect-labeling protocol.

Thus, it is possible to minimize amplification failures, which are a general concern in the cross-species utility of microsatellite markers [ 34].

Linear amplification strategies minimize amplification bias and have had a profound impact on mRNA expression analysis.

Importantly, we only amplified our libraries with 10 PCR cycles (instead of 15 cycles suggested by the manufacturer) to minimize amplification-induced noise.