Not only does this enable quick use of the site - returning to check if one's comments have raised a response could not be easier - but it is also a way of minimising "off-topic" work.
Whilst this could be applied to smaller genomes such as Drosophila, it is possible that even a single sgRNA may be sufficient so long as sgRNA sequences are carefully chosen to minimise off target effects (Bassett et al., 2013; Gratz et al., 2013; Ren et al., 2013), although more rigorous studies will be necessary to establish the extent and nature of off targeting in Drosophila cells.
It absolutely minimises off-target effects".
This highlights the importance of using improved libraries optimised to minimise off-target effects and also demonstrates the utility of post-screen in silico analysis to identify false positive hits resulting from potential OTEs.
BMS-986001 is a thymidine analogue nucleoside reverse transcriptase inhibitor (NRTI) designed to maintain in-vitro antiviral activity while minimising off-target effects.
Farmers retain the income spent on chemical pesticides and fertilisers, minimising run-off pollution to water sources.
In line with this, Slovenian interviewees promote integrative forest management as practised in Slovenia, as it allows for a synergetic balance amongst different forest ES while minimising trade-offs: "The management of forests in Slovenia is a result of a long tradition and forest management planning and it does not have a negative impact on other functions" (Slovenia: conservation group).
The idea was to minimise any off-field stress in the buildup to the World Cup and to prevent any coaches being poached in the event of England flourishing in their home tournament.
A people-focused approach will require concerted action to maximise synergies and minimise trade-offs, but ultimately, it can lead to wins for all – especially poor people and vulnerable groups.
Since NN-DNJ can affect glycosylation of viral proteins we limited the duration of treatment to minimise such off-target effects.
Of particular interest to our group is the ability to better understand and minimise siRNA off-target effects, as such events may either limit the utility of a siRNA being used as a therapeutic agent or compromise interpretation of functional knock-down studies.
