The optimum value of is the one that minimises the expression.
For this reason, minimising the expression in equation (3) selects just one male and one female, i.e., those with the minimum values of a i,Ex.
Use of this unique promoter should minimise interference with the expression of selectable markers or activation-tagged genes resident in Arabidopsis T-DNA insertion lines which typically employ the CaMV35S promoter ([ 36- 38]; I. Parkin, unpublished).
Therefore, we decided to change only the last eleven amino acids of the protein, rather than to make a full knock out strain, in order to minimise changes to the expression strain.
A decision rule could then be adopted to choose that threshold, t*, which minimises the above expression or equivalently which minimises r×P×(1−Se(t))+(1−P)×(1−Sp(t)), where r=(CFN/CFP) is the relative cost of FN compared with FP.
By exchanging nucleotides, which are required for binding of the bacterial RNA-polymerase, but which are of no or little importance for the transcription initiation in the final eukaryotic recipient, it is possible to minimise the heterologous gene expression while maintaining the desired characteristics of the promoter with regard to its function in eukaryotic cells or tissues [ 13].
We found it vitally important to minimise the time intervals between expression of interest and practice visits and between recruitment and initiating the intervention within each practice.
The combined activation of ACC, medial PFC and the angular gyri suggests that once subjects detect an error, they check the expression again to minimise the uncertainty coming from the possibility that it is not the expression that is incorrect but rather that they have made an error [22], [23].
To minimise the effect of missing gene expression levels in each sample, we filtered the training set to include only samples for which there were at least 22 different RQ gene expression values (i.e. 50% of the reporter genes).
As standard immunohistochemistry protocols lower RNA quality (Fend et al, 1999), we optimised a protocol to minimise the K14 staining procedure, enabling expression profiling of the microdissected cells.
