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Then proteins were minimally labeled according to the manufacturer's instructions (CyDye DIGE fluor minimal labeling kit, GE Healthcare, General Electric Company, CT, USA).
BBMV proteins (50 µg per sample) were minimally labeled with Cy3 or Cy5 CyDyes (GE Healthcare) following manufacturer's instructions.
L2 proteins of wild type HPV16 virions were only minimally labeled after denaturation, suggesting that although C22 and C28 are accessible, they are disulfide bonded (Fig. 3C, lane 1).
Interestingly, however, purines were only minimally labeled, presumably because HeLa cells use glycine in the medium as a precursor instead of glucose.
Fifty micrograms of each of cancerous and cancer-free control pancreatic juice protein extracts was minimally labeled with 400 pmol Cy3 or 400 pmol Cy5 fluorescent dye (GE Healthcare).
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SGPs were minimally labelled with CyDye 5 according to the manufacturer's protocol (GE Healthcare) prior electrophoresis, as described elsewhere [ 96].
Another example, which is minimally invasive, uses fluorescein labeled autologous leukocytes to study flow dynamics in humans; however, the authors find evidence of leukocyte activation [ 35].
Someone lying in a hospital bed labelled "minimally conscious state" will be kept on life support indefinitely.
Introduction of a bio-orthogonal amino acid by genetic code expansion, combined with click chemistry, offers novel possibilities for site-specific, minimally invasive labeling.
These results demonstrate that confocal Raman spectroscopy holds promise as a tool for practical, minimally invasive, label-free measurement of microbicide drug concentrations in fluids, gels and tissues.
Minimally, open-label placebo may have potential as a "wait and watch" strategy before prescriptions drugs are prescribed.
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