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Only SNPs that were in agreement with the following parameters were used in the analysis: minimal coverage of 8, minimal variant coverage of 8, minimum variant frequency of 90%, and a p value ≤0.01.
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Single-base substitutions were detected based on minimum total coverage of 10 and minimum variant coverage of 3.
The combined pool sample (23.7 Gb) was processed with a minimal variant frequency of 0.3.
To estimate the minimal coverage for variant detection, we calculated the detection rate at different levels of simulated read coverage.
When we called the variants if SNVs had minimal depth coverage >20× and a quality score >30 in more than 80% of the subjects sequenced, a total of 12,916 variants within 398 genes were identified.
SNVs in the exonic regions of the novel lncRNAs were identified using VarScan (minimal coverage of 8 and variant allele frequency of 0.2), based on the samtools generated pileup input format of the lncRNA regions.
Intriguingly, the story received very minimal press coverage.
Despite his big ideas and cultural cachet, Pinchbeck's new book has received minimal mainstream coverage.
A push by Egyptians for an increased minimum wage has received minimal news coverage.
Before the check-in action, there had been minimal mainstream coverage of the Dakota Pipeline protest.
The calculated minimal nucleotide coverage of the core genome was 58%.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com