Our goal is to present participants' perceptions of GHE relationships and expectations with minimal transformation.
This methodology, being a priori, hypothesis-driven, and with only minimal transformation of data, does not constitute qualitative methodology, but rather a descriptive, topical survey research methodology.
The minimal transformation one can perform consists of deleting one bond and creating another one.
There was some minimal transformation of data such as the obstacles to pain relief.
Let us consider that we are changing 4 bonds, since we aim to find minimal transformations.
The growth deficiency of C. glutamicum IB2103 in CGXII minimal medium was complemented by transformation with plasmid pIB4000 carrying the cloned dtxR gene.
Our results also point out that a single oncogene such as the PDGFB, which according to our data is a weak senescence inducer, might trigger transformation with a minimal number of genetic events in fibroblasts.
One significant observation from this study is that dominant reactions that remove dissolved metals from solution occur within the water column or the oxic and suboxic sediments, with minimal metal transformation within the anoxic sediments over time (and depth).
After transformation with pUA12, pUA13, pUA14, pUA15, and pUA16 plasmids, cells were grown in minimal medium lacking uridine (MM-uri) (0.67% yeast nitrogen base without amino acids, 2% glucose, 2% agar and 100µg/ml of the required amino acids).
Cells were also grown on Edinburgh minimal medium + supplements (ade-nine, histidine, lysine hydrochloride, and leucine) (EMMS-ura; ) at 30°C for 8 days for transformation with plasmids.
