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Finally, 11 BAC clones with minimized overlap were chosen and manually assembled into a contig with minimal tiling path.
The genome assembly was revised using the rice optical map (Zhou et al. 2007) to validate the minimal tiling path.
The Nipponbare genome assembly was updated by revising and validating the minimal tiling path of clones with the optical map for rice.
For the full-genome tiling arrays, we designed a minimal tiling path employing 36-mer oligonucleotides spaced by 10 nt on average to represent the japonica rice genome, based on the TIGR Rice Pseudomolecules release 1 (September 2003) [8].
MTP: minimal tiling path; TE: transposable element.
BACs that matched all consistency criteria were chosen for the calculation of a minimal tiling path.
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The remaining 73,070 BACs were selected from a minimum tiling path representing the physical map of the barley genome16.
This is especially important if minimal tiling paths shall be chosen for sequencing.
Four paradigms have been used to pick minimal tiling paths from FPC fingerprint maps.
The identified clones were superimposed onto the assembled minimal tiling paths and the size of the genes was calculated from the sizes of the individual overlapping clones.
FPC-based physical maps were originally made to assist in clone-by-clone sequencing by identifying minimal tiling paths; indeed, the maize FPC map was used for this purpose as recently as 2009 (Schnable et al. 2009).
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