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In addition, the SsrASTOP mutant that has a minimal tag sequence presented higher sensitivity to oxidative stress upon exposure to paraquat (Fig. 6).
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As shown in the Supplementary Material, the minimal advisable tag sequence length is 15 bp.
We downloaded alignments of CAGE tag sequence with the genome region with a minimal identity of 0.88.
We add 1 to the gradient matrices at each point corresponding to a correct token/tag assignment or a tag/tag transition in the training tag sequence y.
The underlined represents the Flag tag sequence.
To map the DGE tags, the sequenced raw data were filtered to remove adaptor sequences, low-quality sequences (tags with unknown sequences), empty tags (no tag sequence between the adaptors), and tags with only one copy number (probable sequencing error).
Conventional affinity tags are fused as a tag sequence at the DNA level.
If a tag sequence is long enough, the tag matches only one genomic coordinate.
Where j iterates over all possible tag sequences.
After trimming, a total of 140,756 Expressed Sequence Tag sequences were achieved.
Tag sequences were amplified by PCR.
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