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Thus, the minimal supplement of yeast extract significantly promoted the ethanol production as well as the growth of S. cerevisiae.
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Yeast cell seed cultures were grown in 25 ml of minimal medium supplemented with 20 g/l of glucose, and incubated at 30°C for 22 24 hours (130 rpm).
Cells from the 250 mL culture were used to inoculate 1 L of M9 minimal medium supplemented with 100 μg of ampicillin/mL, 1 mL of vitamin solution (23), 1 mL of trace minerals solution (24), and 2 g of unlabeled glucose and 1 g of NH4Cl ([U-N]HscB) or 4 g of [U-C]- d-glucose and 2 g of NH4Cl ([U-C,U-N]HscB).
The populations are maintained by the daily transfer of 0.1 ml of culture into 9.9 ml of fresh Davis minimal media supplemented with 25 μg/ml of glucose (DM25).
Enrichment cultures for the isolation of acid-forming bacteria were grown in minimal medium supplemented with one of the following carbon sources: Na+-dl-lactate, succinate, ethanol, glycerol, glucose or a mixture of amino acids.
For testing growth in liquid cultures, inocula of S. cerevisiae were added to liquid minimal media supplemented with one of the statins or buffer as a negative control.
Anaerobic growth curves were measured in a similar fashion using 50 mL of M9 minimal media supplemented with double the amount of phosphate and with 4% glucose plus 4% fumarate.
T. harzianum was inoculated in a distance of 7 cm against F. solani mycelium in fresh minimal medium supplemented with 0.2% of glucose plates and overlaid with cellophane.
We measured the growth curve of E. coli grown on M9 minimal medium supplemented with different amounts of nitrogen in the form of ammonia (NH4Cl) ranging from a severely limiting level (0.2 mM), to saturating level (18.7 mM).
T. harzianum TR274 plaques were inoculated onto plates containing solid minimal medium supplemented with 0.2% of glucose and overlaid with cellophane at a distance of 7 cm from S. sclerotiorum plaque mycelia.
Inocula of S. cerevisiae cells expressing HMGR genes were added to liquid minimal media supplemented with one of the statins or buffer.
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