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Next, a minimal stringent criterion was used to select miRNA candidates, which ensured that the majority of sequences recovered were known miRNAs.
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These results suggest that mismatch rates are minimal with stringent quality control, and that most genotypic discrepancies are due to technical artifacts rather than the EBV transformation process.
Blast searches of NCBI and Swiss-Prot/TrEMBL databases with full-length TcPRAC sequences resulted in 184 hits from which 111 possess the minimal PRAC stringent MIII* among which 62 hits were directly annotated as 'PRAC', without previous validation of the enzymatic activity.
Nevertheless, it is striking that we obtained these differences despite a stringent minimal read cut-off of 10 reads and 29× (CLC value) medium sequence coverage.
Given the large numbers of SNPs generated that meet these minimal requirements, more stringent parameters can be applied in picking SNP sets for different applications.
Since this definition of cytochrome is minimal, a more stringent definition was created using 26 sequence profiles described in the protein database Interpro as c-type cytochromes.
Interestingly, the FunCat and GO analyses displayed only minimal differences between the stringent and the less stringent conditions, and only in few cases additional categories were found to be enriched (Fig. 4).
This difference was only minimal suggesting that a less stringent stimulus schedule, as used in RISR, is sufficient for successful treatment outcome.
Due to this enhanced background, a more stringent threshold of minimal reads contributing to a peak of 20 was applied.
Perhaps not surprisingly evidence in human for these stringent mechanisms is minimal and some valid questions remain unanswered.
While the minimal intron is universal and stringent in size, the fraction of minimal intron-containing genes appears stable within and across taxonomic scales [36].
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CEO of Professional Science Editing for Scientists @ prosciediting.com