Sentence examples for minimal stem from inspiring English sources

In contrast, neither minimal stem cell growth media nor media plus bFGF produced any undifferentiated stem cells.

These two opposing requirements ultimately lead to optimization of the epithelium turnover rate for a defined organism, allowing maintenance of tissue integrity and organ function with minimal stem cells and cell divisions required.

Undifferentiated stem cells were grown on matrigel-coated wells and cultured according to current methods which included feeding with minimal stem cell media that had been supplemented with 30% conditioned media from Hs27 fibroblast feeder cells.

Undifferentiated H9 hESCs were plated at very low density onto matrigel and cultured for five weeks in either minimal stem cell media or minimal media supplemented with 30% conditioned media from Hs27 fibroblast feeder cells.

After four (4) weeks of culturing H14 embryonic stem cells in minimal stem cell media, and in the absence of conditioned media from fibroblast feeder-cells or bFGF, cells treated with either Anti-MUC1* or NM23 grew faster, formed colonies sooner and resisted the onset of differentiation to a far greater degree than state of the art methods.

SLS identification was performed by using the program rnamotif of the package RNAMOTIF, version 2.1.2 [ 49] according to the following rules: -GU pairing in the stem was allowed -the minimal stem length was 12 bp -loop length could vary from 5 to 100 nt -1 bulged or 1 mispaired base, at least two matches away from the ends of the stem, was allowed.

This 3′-terminal sequence corresponds to the minimal stem-loop structure that has previously been used to program Cas9 in vitro (Jinek et al., 2012).

We showed that a minimal hairpin stem length of 43 base pairs (bp) is needed to obtain two functional siRNAs.

Grade 3 or 4 nonhaematological toxicity was minimal and stem cell mobilisation was not inhibited.

The effect of sample sizes in this comparison was minimal as stem samples longer than 5 mm or leaf blade samples >5 mm showed similar INT, as detailed below.

Minimal root reinforcements at 0.5 m from the stem is about 1 and 11 kPa for 18 and 36-month old plants, respectively.