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The thiol-norbornene system led to hyaline-like cartilage production especially under mechanical loading with positive staining for aggrecan and collagen II and minimal staining for collagens I and X and collagen catabolism.
Nodal nevi are usually located in the fibrous capsule and trabeculae of lymph nodes (but may rarely occur within the nodal parenchyma) and consist of small cytologically bland cells that are devoid of mitotic activity and, on immunohistochemistry, show strong diffuse positivity for S-100 and Melan-A, minimal staining for HMB-45, and a low (<2%) Ki-67 proliferative index.
Consistent with high gene expression levels for the TTR group, strong staining for collagen types I and III (and to some extent collagen type V) was almost exclusively localized to cells at the surface of the lesions and in the surrounding matrix, whereas chondrocytes in the deeper layers and the calcified cartilage showed only minimal staining for the fibrogenic collagens.
There is minimal staining for ERα in the smooth muscle and stromal cells of the isthmus region of the oviduct in both WT and cKO mice, and there was no difference in the intensity of ERα staining in these cell types in the two groups.
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The staining was specific in both tumor and endothelial cells, with minimal staining of surrounding connective tissues.
Results showed minimal staining of VIM was found in SCC25 and HN5, while Tca8113 cells stained strongly.
An arbitrary semiquantitative score was developed to quantify NPM1 immunoreactivity, as follows: 0, from negative to minimal staining (< 10% of cells); 1+, for those tumors showing a weak staining and over 10% of cells; 2+, for those tumors presenting a moderate staining and over 10% of cells; and 3+, for those tumors presenting a strong staining and over 10% of cells.
Immunohistochemical staining for pan-CK revealed that LM38-LP tumors were strongly positive, whereas LM38-HP tumors showed minimal staining or were completely negative (Fig. 6, lower panels).
Staining intensity for Ascl1, NTR2 and NT were scored on a scale of 0 5, where 0 means no staining, 1 means minimal staining and 5 means maximum intensity.
In 28 cases (31%), a minimal staining was observed.
Strong reactivity for FGFR4 was found in 4 cases (7%; Figure 1C), while 51 tumours (83%) displayed intermediate and 6 (10%) only minimal staining intensities.
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