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Minimal sequence requirements for binding were first established using both crystallography and peptide spot synthesis.
In order to determine the minimal sequence requirements for termination-reinitiation in VP2 expresssion, deletions of increasing size were made from the 5' end of the inserted viral information (Figure 1b).
To begin to map the minimal 3' sequence requirements for stable MHC class I expression, we first truncated the 9 Kb genomic fragment used to generate the PD1 transgenic mouse, removing 3.7 Kb of distal intergenic sequences 3' to the PD1 gene, leaving 730 bp immediately downstream of the polyadenylation site.
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However, given that the minimal sequence requirement for termination-reinitiation of BM2 (∼45 nt; [11]) is shorter than that documented in the caliciviruses (69 and 87 nt; [15], [17], [19]), we wished to test whether any of the residues were non-essential.
Introns <30 bp were not found in any of the species analysed, shedding light on the minimal sequence requirement elements used by the cell for conventional splicing functioning.
This sequence is known to be the minimal sequence required for an efficient RNA replication.
To define the minimal flanking sequence requirement at either end for optimal PhoP binding, a series of progressive deletions from either end of RD6 were used as competitors in the EMSA with labeled RD6.
Crystallisation has revealed a conserved core set of elements that define an unusual minimal scaffold of the catalytic domain with remarkably plastic sequence requirements—only a single glutamic acid residue critical to catalytic activity is invariant.
However, the chimaeric protein analysis indicates that the HIF-1 α sequence requirements for CA9 activation are more extensive than the minimal N-TAD, with amino acids 96 390 and 411 574, both being necessary for the response.
Let $f_1, \ldots, f_ r \in I$ be a minimal sequence of generators.
This HCR system has minimal sequence constraints, allowing reconfiguration for the detection of arbitrary target sequences.
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