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We conclude that the α-subunit is the minimal sequence needed for nitrile hydration providing a simplified scaffold to study the mechanism and post-translational modification of this important class of catalysts.
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Our results allowed us to conclude that the minimal DSP1 sequence needed for Corto binding was an intact HMG-box preceded by twelve amino acids (F33 and J11 rather than G81 or L5).
We chose the −77/+57 NXNL1 fragment as the scaffold for these experiments because this minimal promoter sequence contains needed core RNA polymerase II binding elements, since it is active in Y79 and HEK 293 cells (Figure 2A), but it is by itself insufficient for transcriptional activity in the more stringent and biologically relevant environment of retinal explants (Figures 3A and 4).
This sequence is known to be the minimal sequence required for an efficient RNA replication.
Let $f_1, \ldots, f_ r \in I$ be a minimal sequence of generators.
Minimal sequence requirements for binding were first established using both crystallography and peptide spot synthesis.
Furthermore, it has been shown that the last 157 nucleotides at the 3′-end of the HCV strand constitute the minimal sequence required for efficient RNA replication26,27.
This HCR system has minimal sequence constraints, allowing reconfiguration for the detection of arbitrary target sequences.
The 129 sequences that remained had a minimal sequence similarity of ~30% and a minimal sequence coverage of ~50% as compared to hDBC1, complying with previous studies [ 42].
Minimal need; Mild need; Moderate need; Severe need.
The grades will be: Minimal need; Mild need; Moderate need; Severe need.
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