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The minimal sequence coverage depth was set to 20 reads for each nucleotide position.
The minimal sequence coverage was set to a minimum of 10%.
For each nucleotide position, the minimal sequence coverage depth was set to 20 reads.
If not stated otherwise (see Results), a minimal score of 200 and/or minimal sequence coverage of 30% was set as cut-off for low-scoring hits.
The 129 sequences that remained had a minimal sequence similarity of ~30% and a minimal sequence coverage of ~50% as compared to hDBC1, complying with previous studies [ 42].
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We evaluated the minimal sequencing coverage required to obtain a full-length, high-quality genome using in silico assembly experiments.
The minimal sequencing coverage required in F. graminearum to survey accurately the genome to a single base level for recombination events was determined to be 22× fold.
The minimal BLAST sequence coverage was 25 and the minimal percent identity for BLAST hits was 30%%.
Furthermore, our median sequence coverage of 41× (minimal cut-off of 10 reads) is substantially higher than that found in other studies, in which sequencing depth is usually between 6-16× with lower cut-off values than used in this study [ 30, 32, 33, 42, 57].
Clustering of homologous protein sequences was performed using BLASTCLUST [ 71]; we used 40% for the minimal sequence identity (-S option) and 75% for the minimal coverage by alignment (-L option) for at least one sequence (-b option).
Nonetheless, we cannot exclude the possibility that certain peptides might have been lost due to this separation technology, but, based on our overall sequence coverage of hemoglobin, this potential loss appeared to be minimal.
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minimal sequence definition
minimal sequence motif
minimal sequence requirement
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minimal sequence identity
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minimal sequence similarity
minimal sequence change
minimal sequence diversity
minimal sequence depth
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