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Our four UPF0201 structures represent subfamilies N149845 (10077a, b) and N130963 (10077c, d). Figure 2 demonstrates that no residues are absolutely conserved among our four UPF0201 structures, and that there is minimal sequence conservation across the entire archaeal specific family of UPF0201 proteins.
The heterogeneity of Helitron units and the minimal sequence conservation makes curation difficult to automate.
These peptides were chosen so as to have minimal sequence conservation between man and mouse (Supplementary Figure S1) and no similarity with other members of the PLUNC family.
1, 66 This region shows minimal sequence conservation among six species analyzed and can be deleted without adverse effects on receptor function in cultured fibroblasts.
Minimal sequence conservation, short gene lengths, and low complexity sequence can hinder bacteriocin identification, even during gene calling, so they are often discovered by proximity to accessory genes encoding maturation, immunity, and export functions.
Our analyses are in agreement with previous observations; of a conserved adenine anchor [ 16] as higher estimate scores were generally observed with query motifs that included an "A" as a first residue; and of minimal sequence conservation immediately downstream of the seed region.
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Finally, all of these structural characteristics exist with very minimal amino acid sequence conservation, enhancing the significance of the structural findings.
Moreover, we observed enhanced sequence conservation in minimal introns as compared to intronic sequences from large introns and intergenic sequences (Figure 1C).
D2 is not significantly homologous with known structures, but D4 is structurally homologous with Cmr5 and the N-terminal domain of Cse2, although sequence conservation is minimal and the biological implications of the homology are unclear.
Such an event is related to dissimilarities in the spacers, since the 5.8S gene exhibits high sequence conservation and minimal variation in the length of ∼163−165 bp in several species (Baldwin et al. 1995; Manos et al. 2001; Denk et al. 2002; Papini et al. 2011).
Originally identified through conservation of a minimal sequence motif from Drosophila to mammals, PAS domains function as sensors of chemicals and light and act as oligomerization nodes.
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minimal sequence homology
minimal sequence variation
minimal sequence bias
minimal sequence similarity
minimal sequence change
minimal sequence diversity
minimal sequence definition
minimal sequence depth
minimal sequence length
minimal sequence motif
minimal sequence requirement
minimal sequence overlap
minimal sequence identity
minimal sequence effect
minimal sequence present
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