Sentence examples for minimal secondary structure from inspiring English sources

The sequence targeted for capture was derived from the West Nile Virus (WNV) RNA genome and selected on the basis of exhibiting minimal secondary structure formation.

A control construct, [CAA]10/RL, generated a reporter transcript having a 5' UTR consisting of [CAA] tracts and harboring minimal secondary structure with lowered eIF4F dependency [24].

The ssDNA consisted of deoxythymidine nucleotides, which have minimal secondary structure and thus do not require Rpa for cleavage by Dna2 (5'(dto6 to 5' dT 24).

Interestingly, here, the xα2-peptide showed minimal secondary structure propensities in the unbound state, which did not change upon Fyn SH3 binding.

As the secondary structure prediction is one of the input parameters for the de novo modeling, we conducted a set of control modeling calculations, in which minimal secondary structure was provided (see the SSP-full-SERT prediction in Figure 1a).

Arginine residues in the wildtype were pinpointed for conservative replacement by the reactive side chain lysine, since both have similar size and charge; this should result in minimal secondary structure rearrangement [ 11].

Antisense sequences were chosen based on length, location (120 160 nt around the start codon to include promoter and coding regions) [12] and for minimal secondary structures, which were predicted by RNAfold (http://www.tbi.univie.ac.at/RNA/RNAfold.html).html

In the absence of any binding partner, this region shows evidence of minimal helical secondary structure, whereas when bound to either Mdm2 or RPA70, a stronger helical structure is observed.

In brief, 19-to 21-nt-long primers with a 45%55%% guanine-cytosine content were designed to span one or more introns and produce amplicons of 72 120 bp with minimal predicted secondary structure (m-fold).

Primers were 20-21 nt, with a GC content of 40-60%, either overlapped predicted exon-exon boundaries by 3-4 bp or spanned a large intron, and produced predicted amplicons of 55-146 bp with minimal predicted secondary structure (m-fold, [ 91]).

Minimal changes in secondary structure were observed between the engineered peptide and the triblock peptide-PEG-peptide, making it possible that the triblock's multimerization was responsible for this antibody response.