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No minimal sampling time was required to artificially enforce the animal to take a decision.
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This technique is therefore considered rapid when both the minimal sample preparation steps and the APCI-MS headspace sampling time (30 seconds/sample) are taken into consideration.
This minimal sampling frequency is called the Nyquist frequency.
Minimal sample handling and short-time extraction procedures are critical for large-scale metabolomic studies in order to achieve greater reproducibility and sensitivity and to prevent metabolite modification and degradation with time [ 14, 30].
Raman spectroscopy, an analytical technique used to study the vibrational modes in a material, is often employed to distinguish among polymorphs because of its short analysis times, minimal sample preparation requirements, and high sensitivity.
Moreover, unlike the subcellular proteomic profiling [24] that has been explored in biomarker discovery in vivo, the in vivo phage display takes advantages of the minimal sample bias and the real time in vivo binding within the sophisticated tissues and cell structures.
The fluorescence-binding assay described here enables parallel analysis of many samples at one time with minimal sample consumption.
This approach has minimal sample preparation and short run times to achieve high sample throughput.
Miniaturized analytical systems capable of minimal sample consumption and reduced analysis times have been employed to meet this need.
Unlike other methods such as EM or X-ray diffraction, CARS imaging provides high resolution, high-contrast images of the paranodal myelin of individual axons in real-time with minimal sample preparation and no staining, making it possible to follow the dynamic behavior of the paranodal myelin of the same axon throughout the stimulation.
Multiplex testing is a practical approach for detection of the most relevant targets of interest in a single reaction with minimal sample input and shorter turn-around time.
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