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Contrary to traditional respirometry, the goal is detection of bacterial respiration within 5 min in a minimal sample volume.
High-performance size exclusion chromatography (HPSEC), especially with organic carbon detector (OCD), has proved to be an attractive option for NOM characterization, due to its ease of operation, its ability to detect any type of organic carbon bonded species, and also its simplicity of sample preparation and requirement for minimal sample volume (Lai et al. 2015; Hidayah et al. 2016).
ATPS are very appropriate for a POC device, since they are easy to use, can be rapid, and are scalable (to require minimal sample volume).
The ability to maximize this minimal sample volume is essential, as obtaining large samples from critically ill patients, particularly pediatric patients can be difficult.
Technical developments focus on precompetitive issues, such as decreasing minimal sample volume, improvement of sample stabilization and assay sensitivity, reduction of experiment time, data analysis and reduction of costs.
This probe has a minimal sample volume of 35 μL and over 2× greater C mass sensitivity than any commercially available C-optimized cryogenic probe, which translates to well over 4× less measurement time for the same sample and same signal-to-noise ratio.
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It requires minimal sample volumes, the recovery rate is higher than 90% for most nonpolar compounds and 100% of the organic matter sorbed on a stir bar is analysed.
This new technology allows for (1) evaluation of multiple analytes in a single sample; (2) utilization of minimal sample volumes to glean data; (3) reproducibility and results comparative with previous experiments; (4) direct comparison with existing assays; and (5) a more rapid evaluation of multiple samples in a single platform.
Reliable results can be quickly obtained from minimal sample volumes.
Once suitable miRNA biomarkers have been identified and validated, it may be possible to use minimal sample volumes if miRNA abundance is not expected to be low.
Under carefully controlled experimental conditions, multiplexed assays can identify many (96) low abundance candidate proteins using minimal sample volumes (1 μl) [ 11].
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