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Scientists interested in the advantages of NMR metabolomics true quantitation without standard curves, sample preservation and minimal sample processing, and detection of all metabolites with structural information in a single run might be hindered by the startup costs.
Minimal sample processing and rapid ex vivo analysis yields results reflecting the in vivo activation state.
The footprint of the device and simplicity of minimal sample processing with LAMP offers the possibility of point of care, while the microfluidic self-dispensing Gene-Z cards can screen for multiple organism and AR genes in parallel.
Compared to the current cellulose hydrolysis processing regimes for lignocellulosic biomass, which requires fresh enzyme addition with each new batch of substrate, a significant fraction of enzyme activity could be recycled with minimal sample processing, and large decreases in enzyme cost could be achieved.
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[C10,N5]-8-oxodG was not detected in the pilot experiments (1 nmol, in triplicate) spiked with [C10,N5]-dG, which suggests that artifactual formation of 8-oxodG during the sample processing was minimal.
First, the Maxwell 16 requires no additional equipment for sample processing, resulting in minimal sample handling.
SAM may convert to SAH during sample processing; however, every effort was made to minimize delays in processing, and we regarded this to exert minimal effects on our measurements.
Sample processing was completely automated, requiring no ancillary equipment and only minimal technical experience was required.
The Xpert MTB/RIF test also presents a hands-free sample processing technique which can be operated by an individual with minimal training [ 7].
MS requires sample processing.
Fig. 1 The sample processing information.
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