Could it be due to minimal sampling of oriens cells in this account?
This minimal sampling of nematode diversity has implications for our understanding of systematic relationships, as adequate taxon sampling has been identified as a major factor for building accurate phylogenies [ 5].
The additional loss of some non-coding data from the plastid genome along with a few minor changes to intact reading frames within Cuscuta and Convolvulaceae have been reported and roughly mapped on a phylogeny of Cuscuta based on a minimal sampling of taxa [ 20].
For instance, in 2011, rinderpest – a disease affecting cattle and buffalo, and often referred to as the 'cattle plague' – became the second disease-causing virus (after smallpox) to be officially eradicated in the wild through a deliberate global campaign; yet minimal samples of the virus remain stored in high containment facilities for research purposes.
Third, our sample size of 25 per group was higher than the minimal sample size of 17 calculated (on the basis of an independent pretest sample of size 4) to achieve discrimination power.
With an estimated annual hospitalization rate (a primary outcome) of about 6.0%, allowing a maximal deviation of 1.2%, at an alpha error of 5%, a minimal sample size of 1204 subjects was required.
For the planned pair wise comparisons, to detect medium effects or larger (i.e., Cohen's d ≥ 0.5; [ 50]) with at least 80% power and a Bonferroni corrected alpha of 0.025, a minimal sample size of 64 in each group is needed.
Based on our experiences in past research, we presumed a drop-out rate of 10%, implying a minimal sample size of 21/.9 = 24 children in the care-as-usual condition and 36/.9 = 40 in each of the treatment conditions.
This is in agreement with Khatkar et al. [ 15] who suggested a minimal sample size of 400 and 75 for estimation of |D'| and r, respectively.
As a rule of thumb, and even in ideal sampling conditions, a minimal sample size of N = 50 is needed to obtain an acceptable estimate of the population parameter.
