Sentence examples for minimal read length from inspiring English sources

Filter criteria were adjusted to each dataset (qual: quality trimming from 3′ end to a minimal Phred score of 20; l36: minimal read length after trimming of 36 nt; l30: minimal read length after trimming of 30 nt; mp: only paired reads).

We determined the optimal criteria for minimal read length, minimal quality and requirement of mate-pair reads for each isolate based on the different dataset characteristics using FastQC v0.10.0 [ 31].

The first and second mate paired-reads were trimmed with Trimmomatic v0.30 [ 33] using a Phred score of 15 (Q15), a 10 bp sliding window and a minimal read length of 35 bp.

The reads of the three available libraries were filtered (quality > 10 and minimal read length > 31) and SPAdes [ 55] was used with default values to assemble the P. griseofulvum genome.

From this, 41,188 reads were selected based on the alignment length of more than 100 bp and a minimal read length of 1000 bp was used in the de novo assembly of the chloroplast genome of pineapple.

Files containing single-end reads (single left and right reads generated by loss of one of the pair after trimming, single-end raw reads, and mate paired-end single reads without a pair) were merged and trimmed by Trimmomatic with Q15, a 10 bp sliding window, and minimal read length of 70, thus generating one trimmed single-end file.

The block attributes are: number of multi- mapped reads, entropy of read lengths, entropy of read expressions, minimum read length and block length.

The longest M region (28,934 nt) corresponds to a region containing a Ty (yeast retrotransposon) and the rDNA clusters (only one cluster is annotated in the reference genome) on chromosome XII, whereas many M regions exhibit much shorter lengths with a minimal length of 50 nt, limited by the read length resolution.

The number of contigs with read length less than 200 bp was minimal (1% in field pea and 2.2% in faba bean).

However, as sequencing technologies improve critical factors such as read length, it would be prudent to reassess minimal coverage required for accurate assembly.

The average read length was 438 bp.