Missing relative quantifications were imputed for a given protein with a minimal relative quantification observed for that protein across all runs in the same cohort, representing its limit of detection.
We come back to the initial design of the ∇ quantifier by Miller and Tiu, which we call minimal generic quantification.
We present a refinement of minimal generic quantification that brings the expected expressivity while keeping the minimal semantic, which we claim is useful to get natural adequate specifications.
Since galactose and mannose contents were minimal, the quantification provided by the Aminex HPX-87H column was considered accurate.
Böttcher S, Ritgen M, Fischer K, Stilgenbauer S, Busch RM, Fingerle-Rowson G, et al. Minimal residual disease quantification is an independent predictor of progression-free and overall survival in chronic lymphocytic leukemia: a multivariate analysis from the randomized GCLLSG CLL8 trial.
Although some contaminating gDNA remained after column purifications, -RT controls for each gene were more than six cycles greater than the most dilute sample for that gene and thus would have a minimal effect on quantification (Laurell et al. 2012).
Considering its relatively high specificity and low sensitivity, a cut-off near the minimal level of quantification, which may be between 10 and 20 IU/ml, may seem reasonable [ 19].
The genomic fusion site, unique to individual patients and specific for the malignant clone, represents an ideal molecular marker for quantification of minimal residual disease.
Hairpin-shaped primers were demonstrated to have achieved accurate results more consistently than the respective linear primers allowing the specific and sensitive quantification of minimal residual disease of lymphoproliferative disorders with fewer validation procedures and more flexibility on the assay design.
This methodology comprises both melatonin detection and quantification with minimal sample preparation.
