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The read selection strategy, MinimalQ, mentioned above only takes the minimal quality value into account.
Thus, it is reasonable to filter out reads with low minimal quality value.
That is, a pair of reads will be selected if the minimal quality value of the two reads is larger than a given threshold.
Since the base with the lowest quality value is most likely to cause misassembled contigs, the MinimalQ strategy identifies the minimal quality value of each read, and sets a threshold of selecting reads with minimal quality value no smaller than the threshold.
As shown in Figure 1a and Figure 2a, although both the E. coli and B. cereus datasets have bases quality values of 80% or above at Q30 (Table 1), the E. coli dataset has a peak at minimal quality value 9 with 51%, and the B. cereus dataset at minimal quality value 8 with 26% in terms of reads' minimal quality statistics.
The result generated by MinimalQ or MinimalProductQ program is a list of records, and each record contains a read followed by its corresponding minimal quality value (MQV) or correctness score, respectively.
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Consequently, PEAR includes the option to trim unmerged reads that contain at least two consecutive bases with quality scores lower than a user-specified minimal quality score value.
Since the Illumina platform generates a quality score for each locus, we hypothesized that it would be possible to establish a minimal quality cut-off value that would eliminate most of the erroneous genotypes.
In addition, the overall sequence quality of the genome was further improved by using the following criteria: (1) two independent high-quality reads as minimal coverage, and (2) Phred quality value = Q40 for each given base.
Composition data fulfilling the minimal quality requirements and used to produce the mean content values are immediately accessible to the general public on the website after update.
Reaction efficiency of 0.9 and the correlation coefficient (r) values of 0.99 were assumed as minimal quality standards for data acquisition and melting curves were also analyzed to confirm specificity.
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