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A microcolumn is presented containing a variable axis lens optimized for large scan fields and pixel numbers with minimal probe sizes for the deflected beam.
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For 215 genes, two or more transcripts were covered by the newly defined transcript-specific probe sets with a minimal probe set size of 1 (Table 2).
A minimal probe set size of 3 reduced the number of genes to 141 (136 genes with 2 transcripts, 4 genes with 3 transcripts, 1 gene with 4 transcripts).
(The horizontal sample feature size was approximately a tenth of the nominal probe sizes).
A number of factors affect digitizing accuracy, such as the travel speed of the probe, pitch values, probe angles (part orientations), probe sizes, and feature sizes.
The minimal probe set spanned different non-overlapping regions of the gene (Fig. 2A).
Probe sizes were 50 75 mer in length and median probe spacing was 200 bp.
e Probe size.
Moreover, the probe size can be optimized with respect to tumor size and location via repeated simulation anlysis with several probe size.
Probe size ranged between 26 and 30 bp.
Always match the probe size to the snake (the smaller the snake, the smaller the probe).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com