Sentence examples for minimal plates from inspiring English sources
Plate everything on galactose minimal plates containing chloramphenicol or kanamycin if pBALU-ext was used for extension.
However, for four auxotrophs – ΔserB, ΔgltA, ΔilvA, and Δfes – colonies formed on minimal plates after several days of incubation (Figures 2A & 2B).
To confirm that the colonies observed on selective media resulted from the uptake of a novel gene and not from an adaptive mutation on the chromosome, colonies were isolated by restreaking, and plasmid DNA was purified; this DNA was then used to transform naïve cells of the same auxotroph, and the transformed cells were once again spread on both LB and minimal plates.
WS stability was found to be very similar on 25 mM glucose (Gluc) and 40 mM succinate (Suc) minimal plates at 28°C (%WS: Gluc, 28.5±6.5%; Suc, 20.2±13.5%, Welch ANOVA, F1,2.8833 = 0.3066, P = 0.6198), indicating that the WS maladaptive pressure to revert to SM-LR was similar when utilising either carbon source.
After bombardment, the cells were spread onto MA5 minimal plates solidified with Noble agar.
After 2 days at 32°, we isolated single diploid colonies from the minimal plates and restreaked them to single colonies on fresh minimal medium.
The smallest pillar diameter even produced the worst minimal plate height (4 μm).
The minimal plate height decreases from 1.7 to 1.2 μm when going from 4.80 to 3.81 μm diameter pillars, but as the dimensions are further reduced, the minimal plate heights rise again to values around 2 μm.
The obtained results strongly deviated from the theoretically expected trend, wherein the minimal plate height (Hmin) would reduce linearly with the pillar diameter.
(C) BL21 DE3) cells harboring the wildtype and PRK2021 plasmids were streaked on M9 minimal plate containing 50 μg/mL kanamycin, 1% (w/v) xylose, 0.05% (w/v) casamino acids for PRK expression.
The nkr mutant was obtained by selecting colonies grown on nickel-containing minimal plate.
