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As a result, it trims the various sequences at an approximately equal length, reducing the length of the longer sequences; this can therefore be problematic when taxonomy is assigned using a minimal percent of similarity with the reference sequences.
The minimal BLAST sequence coverage was 25 and the minimal percent identity for BLAST hits was 30%%.
ESTs: Expressed sequence tags; WGS: Whole genome shotgun; MIL: Maximal intron length; MLC: Minimal length coverage; MPI: Minimal percent identity.
In PRICE assemblies, the minimal overlap was set to 30, and the minimal percent identity to 95 and 85 for Illumina and 454 data sets, respectively.
Secondly, we counted for each region of a predicted SNP position the number of mapped reads that show a minimal percent identity of either 90%, or of 100%.
The pipeline selects 'guide' transcripts and their aligned regions using maximal intron length (MIL), minimal length coverage (MLC) and minimal percent identity (MPI) as parameters.
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A minimum percent nucleotide identity threshold of 70% and 20 bp as a minimal length criterion were considered significant in VISTA [ 18] for our analyses.
Meanwhile, the gap between gay men and lesbians appeared minimal: 89 percent versus 86percentt, respectively.
The peptides were limited fully tryptic with a maximum of cleavage of 2. Other parameters included the following: carbamidomethylation of cysteines, static; iTRAQ modification on the N-terminus and lysine, differential; ±20 ppm for precursor ions; ±0.7 Da for fragment ions; minimal matched percent scored peak intensity, 50%.
In addition, almost ninety percent had minimal eating disorder psychopathology at the end of treatment, despite the weight gain.
Incubation of 60 and 200 nm anionic NPs with BSA resulted in minimal changes to the percent α-helicity, 71 and 63%, respectively.
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