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Whole genomic DNA was isolated from bacteria that were subjected to minimal passage, using Qiagen Genomic‒tip 100/G columns and the Genomic DNA Buffer Set (Qiagen).
Seven L pneumophila isolates (three clinical and four environmental) were obtained from the reference laboratory where they had been stored at −80°C with minimal passage since the outbreak.
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As anticipated, pooled clones with minimal passaging time did not show any transgene rearrangements.
Playing 18th-century instruments with gut strings, the ABO is "hardcore", says Richter, whose own work is Vivaldi for the 21st century, with long looping rhythms, minimal shimmering passages stretching time.
The sequenced strain was obtained from the original stock kept at the Center for Vaccine Development and was subjected to minimal laboratory passages.
All in vivo experiments were undertaken using cells established from the same KHT stock tumour after minimal in vitro passaging (<6 <span class="lh lhl">passages).
The dictum was thus in the beginning, based on pharmacokinetic studies [7, 19] that sumatriptan had only minimal or no passage within the central nervous system.
The NB TICs were isolated and propagated in serum-free sphere-forming conditions optimized for neural stem cells growth and maintenance and are used with minimal in vitro passaging.
Winds during Don's passage were minimal, with a peak wind gust of 41 mph at Waldron Field.
On 18 December Tymoshenko was once again elected as Prime Minister (supported by 226 deputies, the minimal number needed for passage), heading the second Tymoshenko Government.
(36) We found that maximal labeling was achieved by the third passage with minimal increases in labeling with additional passages (Table 2).
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