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Images were segmented using a local maximum seeded watershed approach (LMSW), identifying single cells by nlsGFP intensity profile and clusters by the distance between cell intensity centroids according to fixed criteria (minimal particle width: 2.5 μm, maximum particle width: 10 μm, maximal distance for grouping: 8 μm) (detailed method in Zhu et al., 2010).
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Minimal particle radius has been found as 2 nm.
After pretreatment, the particle width range was reduced.
SEM images show that the particle width range of untreated MCC was ~25 – 60 μm (Additional file 4: Figure S4a).
As shown by the scanning electron microscopy (SEM) images, the average particle width of untreated bagasse was ~250 – 500 μm while after GC pretreatment the width was reduced to ~40 – 150 μm (Additional file 2: Figure S2a), similar to, if not smaller, than the particle width range of fibres from EC pretreatment (Additional file 2: Figure S2b).
Pretreatment by glycerol reduced biomass particle width to 60 – 120 μm (Additional file 2: Figure S2c) while pretreatment by EG partially defibrillated biomass fibres (with a width range of 20 – 30 μm) (Additional file 2: Figure S2d).
Taguchi-based gray relational analysis was used in the optimization of the cutting parameters for minimal burr width (for up and down milling operations) and surface roughness (Ra).
Minimal stencil width discretizations of combined mixed and non-mixed second-order derivatives are analyzed with respect to accuracy and stability.
The cortical bone static measures included cortical bone area, marrow area (medullary cavity), average cortical width (from periosteal to endocortical surface in cross-sections), and minimal cortical width.
Minimal interval width was set to 0.5 Da for both analyses.
The Top Hat baseline with 10% minimal baseline width was used for baseline subtraction.
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