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Seedlings were then transferred to buckets containing 2000 mL of sterile nutrient solution for solution culture.
Plants were allowed to adapt to this nutrient solution for 2 weeks.
The seedlings were grown with nutrient solution for 22 d after germination.
Control plants were continuously supplied with 250 μM Pi in nutrient solution for 43 days.
Seedlings were then transferred to 0.25 strength nutrient solution for one day.
L. leucocephala plants were grown in vermiculite flasks with N free Fahraeus nutrient solution for 40 days and P. vulgaris plants in agar flasks with the same nutrient solution for 14 days.
Plants with healthy and uniform shoots of almost equal morphological characteristics were immersed in nutrient solution for three weeks.
Healthy and vigorous seedlings were selected and grown in aerated optimum-nutrient solutions for 15 d [ 98].
The half Hoagland's nutrient solution without Al was used for control plants.
Algae can interfere with the plant growth by competing for nutrients, reducing the solution acidity, competing for oxygen in the nutrient solution at night, producing toxic products through its decomposition and creating odours.
They then pulsed a vitamin and nutrient solution through the tube, imitating the conditions for artery growth.
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