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The calculated minimal nucleotide coverage of the core genome was 58%.
GST encoding transcripts were aligned to the reference C. sinensis genome scaffolds using GenomeThreader [ 58] with 80% minimal nucleotide coverage and 80% sequence identity.
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This standardized NGS pipeline demonstrated 100% sensitivity and 100% specificity, uniformity, and high-depth nucleotide coverage per sample (approximately 7000 reads per nucleotide).
Y-axis denotes the nucleotide coverage.
With lesion-modified templates, both polymerases exhibited minimal nucleotide selectivity.
With this strategy information about nucleotide coverage is maintained which is important for reliable SNP discovery.
Thus, the average nucleotide coverage per library was 9.1 ± 0.4 × 10.
Nucleotide coverage queries and read depth analyses were performed using SAMtools [ 32].
For each nucleotide position, the minimal sequence coverage depth was set to 20 reads.
The minimal sequence coverage depth was set to 20 reads for each nucleotide position.
Intriguingly, the story received very minimal press coverage.
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