Our gene properties associate most strongly with rich medium HI, with weaker relationships between gene properties and HI in minimal, nitrogen-limited, carbon-limited, phosphate-limited, and grape juice media.
For minimal media nitrogen was supplied as either NaNO3 (nitrate) or ammonium tartrate (10 mM).
We then scooped up the mated cell mixture and spread it on minimal yeast nitrogen base (YNB) medium to select diploids.
Most of these studies focused on pre-penetration stages, such as conidiation and appressorium formation, and on growth in a variety of in vitro conditions, including complete medium, minimal medium, nitrogen starvation, and rice cell wall medium.
AU: Arbitrary unit; FL: Fluorescence light; kb: Kilobase; MM: Minimal medium; N: Nitrogen; PCR: Polymerase chain reaction; TAG: Triacylglycerol; TLC: Thin layer chromatography; TAP: Tris-acetate-phosphate.
In nitrogen-replete conditions, Gln3 is cytoplasmic and Gln3-mediated transcription minimal, whereas in nitrogen limiting or starvation conditions, or after rapamycin treatment, Gln3 is nuclear and transcription greatly increased.
The strains underwent initial adaptation on the residues at 1.5% solids in 10 mL of 0.1% w/v nitrogen minimal media for 24 h.
After the initial adaptation phase, all cells and residues were transferred to 150 mL of 0.05% w/v nitrogen minimal media and 1.5% solids loading, that was sterilized by autoclaving for 20 min at 121 °C.
The mycelia were then washed and subsequently transferred to a flask containing minimal media without nitrogen and grown for an additional 4 hr (see Materials and Methods).
Total RNA was purified by acid phenol extraction from cells cultivated in either complete (YPD) or minimal (W0: yeast nitrogen base 6.7 g/l, d-glucose 20 g/l) media and harvested at either early log or stationary phases.
