Exact(3)
We use integer programming methodology to model and solve the problem of computing the minimal mixture of oligonucleotides required to induce an arbitrary target probability over the 20 standard amino acids.
For instance, a minimal mixture of cellobiohydrolases (CBHI and CBHII) and endoglucanase (EGI) from T. reesei and β-glucosidase from A. niger is sufficient to efficiently degrade pure cellulose (Sternberg et al. 1977; Rosgaard et al. 2007a).
To test whether PCB 126 is required to be present in a minimal mixture of PCBs to activate the DR4-tk-LUC construct in GH3 cells, we tested the same PCB combinations for their ability to drive luciferase activity from the DR4.
Similar(57)
There is something utterly compelling about that mixture of minimal dairy fats, vegetable oil and air.
In minimal medium with the mixture of D-galacturonate plus glucose, C. glutamicum WT pHexA)(pVWEx1) grew with a growth rate of 0.25 ± 0.02 h−1 and co-utilized glucose with D-galacturonate (Figure 2).
Moreover, it was required to pre-cultivate the strain in minimal medium with a mixture of 50 mM glucose and 50 mM of either D-galacturonate or D-glucuronate as carbon source.
No more hard techno, more a mixture of minimal house and ambient jams.
The MCs were plated on 100-mm plastic dishes (Nunc) that were maintained in B medium (a 3∶1 mixture of minimal essential medium/F12 modified with trace elements) supplemented with 1 mM glutamine, penicillin at 100 units/ml, streptomycin at 100 µg/ml, and 10% fetal calf serum (Irvine Scientific).
All resident communities were inoculated to 20 different resources environments (total of 620 individual populations), each of which were composed of OS minimal medium supplemented with a random mixture of 20 carbons selected from the total pool of 48 resources (supplementary table 4).
The cells were re-suspended in 6 ml of a mixture of Eagle's Minimal Essential Medium with 2 % Dimethyl Sulfoxide and stored in tubes of 1 ml at −80 °C.
Contrary to previous reports, E. coli produced H2 after aerobic growth in minimal medium without inducers and with a mixture of organic acids dominated by butyrate.
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