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The microplate alamar blue assay (MABA) and the broth microdilution method were used to determine the minimal inhibitory concentration (MIC) and minimal microbicidal concentration (MMC) of the above samples.
All the synthesised peptides were tested for their in vitro microbicidal activity against S. aureus (American Type Culture Collection (ATCC) 12600) and P. aeruginosa (ATCC 15442) using a minimal microbicidal concentration (MMC) assay as previously described (Haversen et al. 2010).
The microbicidal effect of centrocin 1 heavy chain (CEN1 HC-Br), its debrominated analogue (CEN1 HC), the C-terminal truncated variants of both peptides, i.e. CEN1 HC-Br (1 20) and CEN1 HC (1 20), as well as the cysteine to serine substituted equivalent CEN1 HC (Ser) was evaluated using minimal microbicidal concentration assay.
The MHB and Mueller Hinton Agar (MHA) were used to determine the minimal microbicidal concentration (MMC) of the active samples.
The liquid microdilution assay was used in the determination of the minimal inhibitory concentration (MIC) and the minimal microbicidal concentration (MMC), against seven bacterial and one fungal species.
The microplate alamar blue assay (MABA) and the broth microdilution method were used to determine the minimal inhibitory concentration (MIC) and minimal microbicidal concentration (MMC) of the above extract and compounds on a panel of bacterial species.
Similar(54)
minimum microbicidal concentration.
The lowest MMC (minimum microbicidal concentration) observed was 39.04 μg/mL for C. tropicana against MSBE.
In determining the minimum microbicidal concentration, E. arvense L., P. granatum L., and S. barbatimam Mart.
After determination of the Minimum Inhibitory Concentration (MIC), 100 μL of this concentration and one above and one below this concentration were seeded onto BHI or Sabouraud-dextrose agar plates to determine the Minimum Microbicidal Concentration (MMC) of the extracts.
Table 3 Microbicidal activity of recombinant ABF-2 Organism BC 50 μM) Gram-positive bacteria 0.01 Staphylococcus aureus (ATCC6538P) Gram-negative bacteria 0.1 E. coli (K12) Microbicidal activity was assessed as the 50% microbicidal concentration (BC50).
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