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Through fed-batch fermentation using resting cells, over 1.1 g/L 1-hexadecanol can be produced in glucose minimal medium, which represents the highest titer reported in yeast to date.
This strain was able to mineralize 400 mg/L of MG in minimal medium, which was higher than previous reports made by Parshetti et al. (2006) in which K. rosea could mineralize 50 mg/L of MG in semisynthetic medium.
As CA production of the strain ATCC 1015 was evaluated by glucose, it was tested in a glucose-based minimal medium, which only achieved 0.10 ± 0.12 g/L CA (Andersen et al. 2011).
C. crescentus does not produce any ElpS-3Flag when grown in minimal medium, which is surprising if we consider that minimal medium has lower nutrient content than poor medium.
The T3SS genes of phytobacteria are induced in the plant apoplast, when in close contact with host cells or in nutritionally poor synthetic T3SS-inducing minimal medium, which is thought to mimic conditions within the plant apoplast [1], [2], [4].
The lignocellulosic hydrolysate was added to a minimal medium which contained 0.3 g/l yeast extract.
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The different nematode growth conditions were: i) hatched and matured in minimal S medium, which has little nutritive value and induces starvation and increased sensitivity to various food odors [9], ii) grown in S medium supplemented with heat-killed E. coli, or iii) grown in CeHR medium, a rich food source free of bacteria.
Parasites were suspended in Eagle's minimal essential medium, which resulted in 1 × 10 parasites per millilitre.
Each sample was placed in 2 mL minimal essential medium, which was supplemented with penicillin (2000 Units/mL) and streptomycin (2000 Units/mL).
Explants of tracheas from hamsters were cultured in either a chemically defined minimal essential medium, which maintains a differentiated epithelium, or a nutritionally complex medium, which encourages the development of squamous metaplasia.
HepG2 cells from the Riken Cell Bank (Tsukuba, Japan) were cultured in the minimal essential medium, which includes Earle's salts, non-essential amino acids but not - L-Glutamine (MEM, Invitrogen Corporation), supplemented with 10% fetal bovine serum (Invitrogen Corporation), 50 U of penicillin/ml and 50 μg of streptomycin/ml.
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