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The minimal medium was used, in contrast to a more nutrient rich one, to minimize the amount of volatile compounds originating from the medium.
A set of fermentation strategies based on this minimal medium was developed and the lipid content was raised to 51%.
In our first test, the turbidostat feed of cultures grown in M9 minimal medium was abruptly switched to richer LB medium.
The initial pH of the minimal medium was 6.8.
However, low culture densities achieved in the minimal medium was not suitable for an industrial production of SAM.
Also Chaetomium senegalense and Talaromyces byssochlamydoides grew better when the cellulose minimal medium was supplemented with yeast extract.
The minimal medium was used in all experiments so that yeast growth rate was at its minimal and cells inside the beads were assumed to be uniform.
Ceftazidime (Sigma-Aldrich, St. Loius, MO, USA) at 50 µg/ml concentration was used as the positive control and minimal medium was used as the negative control.
In the transformation, the minimal medium was supplemented with 1.2 M d-sorbitol, 10 g l−1 of d-glucose and 20 g l−1 of agar and the pH was adjusted to 6.5.
For testing the phenotype, the minimal medium was supplemented 20 g l−1 agar and 9 g l−1 l-rhamnose, or in the liquid cultivations with 22 g l−1 l-rhamnose.
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Because transition metal concentrations in minimal medium were substantially lower than in LB, ICP-MS measurements of minimal media samples employed the standard addition method.
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