Sentence examples for minimal medium and from inspiring English sources

Then, 100 ml of the culture were added to 50 ml of yeast nitrogen base minimal medium and grown to OD600 0.5 at 30° in a shaking incubator.

To create growth conditions in which Spot 42 was highly expressed in the wild-type strain, the cells were cultured in ASMM minimal medium and then stimulated by adding glucose as the main carbon sources.

Our results were confirmed by growing S. sanguinis in minimal medium and by double-knockout of paralogous or isozyme genes.

The resultant clone was pre-cultured for 2 d at 28 °C in SC minimal medium and then cultured for 2 d at 20 °C in the SC minimal medium containing 2% (w/v) galactose substituted for glucose.

The total cellular concentration of each element was calculated assuming a maximal cellular volume of 1.8 m m 3 for cells grown in minimal medium and 3.5 m m 3 in LB medium ( 23, 31 ).

Provision of rare tRNAs has increased RibU production by 7.7-folds and 2.86-fold in minimal medium and rich medium, respectively, while MtrA levels were increased by 1-fold in minimal medium.

Glucose was autoclaved separately from the minimal medium and later added to the media at the concentration of 20 g/L.

Furthermore, the strain was capable of growing well in both rich and minimal medium and producing its mesophilic lipases and cellulases without inducers.

Three clones of each strain verified with PCR were used to inoculate 14 ml sterile falcon tubes containing 2 ml minimal medium and cultivated at 30 °C and 200 rpm for 24 h.

In brief, fungal inoculum was grown aerobically in a 250 ml Büchner flask containing 50 ml minimal medium and 1% (wv-1) alkali treated cellulose and 0.15% (wv-1) wheat bran as carbon sources.

Resulting single clones were inoculated into liquid SD-URA minimal medium, and incubated over night.