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MSCs are plastic adherent cells expressing the minimal marker set of CD90, CD73 and CD105 and absence of CD14, CD79, CD34, CD45, and HLA-DR [1], [2].
However, they used a high-sensitivity assay that enabled detection of minimal marker elevation.
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The choice of the antibodies used was based on the minimal surface marker panel proposed by the International Society of Cellular Therapy [ 12].
Although hMSC have a common minimal expression marker profile of surface receptors, additional distinct surface molecules and metabolic alterations can be detected between different hMSC populations which may determine their functional diversity within the different tissues.
Expected BMD loss was perhaps more acceptable (~1%) from 60% suppression, but because of relatively minimal bone marker changes from this suppression level, it is unlikely that they would serve as useful biomarkers to differentiate efficacy and tolerability.
The 191 SxG offsprings have been previously genotyped for 97 SSR markers to cover the genome with minimal inter-marker space (maximum of 10 cM) with the most informative polymorphisms (priority given to 1 1 1 1 segregating markers).
Among the 42,846 available SNPs, only 8,342 SNPs (selected to achieve a minimal inter-marker distance above 200 kb) were included in the analysis and marker position information was considered.
The southwest Pacific ICEMR is developing algorithms to identify the most informative markers so that minimal numbers of markers can be developed as a parasite "barcoding tool"; other ICEMR sites are expected to follow suit.
More recently body fluids as source for non-invasive or minimal-invasive markers have become more important.
A 4.2-L filtration volume, the use of 2.5-μL of undiluted DNA extract in qPCR, and the minimal theoretically detectable marker concentration per reaction defines the threshold of detection that is shown in Figures 2 and S1.
The maximal squared Pearson correlation (r) between rs9804659 and any of the CNV markers in CNVR 11q11 was 0.65, while r was 0.53 between rs9804659 and the best associated CNV marker (minimal P-value in the family-based discovery sample; Table 3).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com