Sentence examples for minimal manipulation to from inspiring English sources

First, we wanted to determine whether the observed regional gradation of expression of cell surface markers of pluripotency was reflected in patterns of pluripotency gene expression, and second, we wanted to compare these results, obtained from cells subjected to minimal manipulation, to those from cells that were separated by flow cytometry in subsequent experiments.

PBMCs were used as they are obtained through non-invasive procedures and require minimal manipulation to isolate.

Moreover, considering the minimal manipulation of BM to select CD133+ cells and the absence of an extensive expansion of cells, mycoplasma testing will be omitted from standard release criteria in our current protocols (see FPC2 Supplemental Table 2), thus saving 1 × 10 cells+ cells per batch injectable to the patient.

- are easy to dispense and: they require minimal manipulation by health professionals and parents prior to use which minimises the risk of errors.

The advantages of this assay include the fact that it requires minimal manipulation of the sample compared to assays that rely on extraction of nucleic acids and nested PCR methods.

A gfp reporter was inserted seamlessly at specific points in each gene, with or without subsequent minimal manipulations, so as to assess the contributions of different transcripts to the complete expression pattern of each gene.

The concept of "minimal manipulation" and of "heterologous use" are introduced to describe currently used criteria.

Fourth, sample preparation after solubilization was very brief (<30 min) with <span class="lh">minimal manipulations, in contrast to the multistep purification that resulted in partial loss of nascent chains (Park et al., 2014).

DOI: http://dx.doi.org/10.7554/eLife.02001.006 10.7554/eLiFigure01.007 Figure 2 figure supplement 1. Minimal manipulation of reconstituted episomes minimizes the generation of deletions.

This spiritually utilitarian approach to art also makes me think of certain Yoruban assemblages that, through minimal manipulation of the merest scraps of natural materials, assume moral and magical properties.

To bypass these technical difficulties, we turned to a model of peritonitis where many inflammatory cells are recruited to the peritoneal cavity upon thioglycollate administration and are easily isolated with minimal manipulation.