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DOI: http://dx.doi.org/10.7554/eLife.02001.006 10.7554/eLiFigure01.007 Figure 2 figure supplement 1. Minimal manipulation of reconstituted episomes minimizes the generation of deletions.
The assay system involves minimal manipulation after assay set-up, facilitates automated read-out and minimizes risks associated with hazardous viruses.
These facts create a strong case for finding minimal manipulation.
"I believe in minimal manipulation," he told me not long ago.
"It feels absolutely great to be making more-natural wines with minimal manipulation.
The concept of "minimal manipulation" and of "heterologous use" are introduced to describe currently used criteria.
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These minimal manipulations had little or no effect on the amino acid selectivity of TyrRS, since the CP1 region of TyrRS is remote from the amino acid-binding site [12].
Key points included minimal manipulations with the grafts ("no-touch" technique) and prevention of extensive dilation of the conduits.
Despite lower dye incorporation and global fluorescence of the array, the new direct random method provided excellent reproducibility across replicates, possibly because of the minimal manipulations.
A gfp reporter was inserted seamlessly at specific points in each gene, with or without subsequent minimal manipulations, so as to assess the contributions of different transcripts to the complete expression pattern of each gene.
Fourth, sample preparation after solubilization was very brief (<30 min) with minimal manipulations, in contrast to the multistep purification that resulted in partial loss of nascent chains (Park et al., 2014).
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