The results demonstrated that no-observed-adverse-effect level (NOAEL), lowest-observed-adverse-effect level (LOAEL), maximal tolerance dose (MTD), and minimal lethal dose (MLD) were respectively 5, 6, 30, 35 mg/kg for i.v. administration of dipfluzine hydrochloride.
Groups of 10 animals received a single i.p. dose of hyperimmune serum (using the HS-np dilution) administered 1 h prior to i.p. inoculation of the bacterial minimal lethal dose.
with different inocula in Todd-Hewitt broth: 1×106, 5×106, 1×107, 5×107, 1×108 cfu/ml (spectrophotometrically measured) to determine the minimal dose that produced a 100% mortality rate over a 7-day follow-up period (i.e., the minimal lethal dose).
The extreme toxicity requires ultrasensitive diagnostic assays with detection limits well below the minimal lethal dose to allow timely administration of therapeutics.
To find the minimal lethal dose (MLD), serial dilutions of P. aeruginosa (10, 10, and 10 CFU/mL) were injected intraperitoneally (i.p).
These concentrations are comparable to those in a study of L. casei, a bacterium commonly used in probiotics, reporting that the 50% lethal dose in mice was 9 × 10 CFU/kg bw, whereas the minimal lethal dose in rats was 40 × 10 CFU/kg bw [ 32].
