Exact(4)
Radical mutants of individual residues within the center of the minimal leader region revealed S30F had no effect but mutants L29R and E31R did not allow processing of the PatE2 to completion.
Processivity (heterocyclization of the second or internal cysteine of the core peptide), although very sensitive to changes in the minimal leader, is insensitive to changes in the core peptide flanking regions.
A synthetic peptide with the first 25 residues of PatE2 deleted (Δ25PatE) retains the most conserved leader region, residues 26-35 (denoted "minimal leader"), and was processed by TruD only slightly more slowly than PatE2 (judged by MS; Figure S9).
As multiple core peptides share a single leader, the signatures flanking the core peptide can be mutated extensively and insertion of residues between the core and leader can be tolerated; we conclude that the spatial relationship between the minimal leader and target residue is highly flexible.
Similar(56)
Though June 18 Although the sculptor Donald Judd is considered a leader of Minimal art, he had little use for art movements and considered his own work more than "sufficiently complex," to use one of his favorite crypticisms.
For example, if the maturity level of the employees is high, the model suggests a delegating style of leadership with minimal guidance from the leader, whereas the leadership of less mature employees should be of a more supportive kind [ 42].
But foreign affairs analysts said that Obama's leverage with the European leaders is minimal.
Such an approach would put at risk whatever minimal trust the two leaders have built up over the past year.
A pure counterterror approach had, in fact, been the Bush Administration's policy for years: kill or capture terrorist leaders, with minimal support for political institutions in Kabul and Islamabad.
A reminder of the minimal effort national Republican leaders have made came on Thursday when Senator McCain, coaxed by some New Yorkers, released a relatively rare endorsement of a New York Republican candidate: J. Christopher Callaghan, whose once-long-shot bid for comptroller has been invigorated because of a scandal that has tainted the Democratic incumbent, Alan G. Hevesi.
Actually, you've mostly sold to the 20-something kids of my IT leaders with minimal human interaction against virtually no competition.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com