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Currents produced by Cav2.3/Cavβ2a did not run-down in the presence of 5 mM intracellular NaCl, Na2-ATP, Tris2·ATP or in minimal intracellular solution.
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Pipettes contained normal intracellular solution.
When filled with intracellular solution, the resistance was 2.0 3.0 MΩ.
In a subset of experiments, 50 μM ZD7288 was added to the intracellular solution from a 5-mM stock solution.
All experiments were performed with 5 mM EGTA in the intracellular solution.
The intracellular solution was the same as used for the noise analysis.
Pipettes had a resistance of 2 4 MΩ when filled with intracellular solution.
When filled with the intracellular solution, the electrodes had resistances of 3-5 MΩ.
Patch clamp recordings were made at room temperature (21 24°C) using CsCl-based intracellular solution.
The pipettes had a resistance of 1.5 2.5 MΩ when filled with intracellular solution.
The liquid junction potential for the intracellular solution is −11 mV.
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