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After preprocessing, detection template was designed based on the local minimal intensity, and a binary image containing segmented boundaries was obtained by applying logical rules, thinning and filtering.
Pictures were taken in the GFP channel with minimal intensity and exposure time (20 50 ms) at 20 min intervals for at least 24 h.
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DTA files were generated for MS/MS spectra that both reached a minimal intensity, 1.10e3, and a sufficient number of ions, 10.
The m/z ranges were from 800 to 4,000 with peak density of maximum 30 peaks per 200 Da, minimal S/N ratio of 5, minimal area of 10, minimal intensity of 150, and 200 maximum peaks per spot.
The RT-PCR amplification of samples of cell culture supernatants provided amplification bands with minimal intensity for FMDV WT and M296I subjected to FUG-500 treatment, confirming the drastic effect that the treatment had on viral load.
The theoretical lateral domains are defined by considering the domain where the actin intensity is superior to the minimal value plus 50% of difference between maximal and minimal intensity.
Again, precision parameters β1 (at full intensity) and β0 (at minimal intensity) are provided by the user (in percent).
The peak at 700 nm shows a maximum intensity for 80° polarization and a minimal intensity for 0° polarization.
The cycle times were set to 5 s, and within this 5 s interval, the most abundant ions per scan were selected for either CID or EThcD and detected in the orbitrap with a resolution of 15K with an AGC target of 5e and a minimal intensity of 5000.
b SWI, minimal intensity projection (minIP; 10 mm) image: cortical and subependymal veins are well displayed.
Approaches to this issue have included requiring that each gene have a minimal intensity across all conditions [ 12, 15], and that genes without significant changes be eliminated from further analysis [ 17].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com