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Since a short incubation time was desirable to minimize cell death and damage to short-lived macromolecules, this minimal incubation time is considered an important advantage.
Interestingly the minimal incubation time at the restrictive temperature, required for the elimination of contractions, was almost identical for both the ts20 and the ts41 cells (8 hours) and for MG132 (7 hours).
We found that the minimal incubation time required for visualizing fluorescent signals is 6 h.
The in-house developed ELISA [ 22] for the detection of IL-1 β requires minimal incubation time and is based on Sandwich method of antigen detection [ 24, 25].
The EUCAST stresses that there are currently no validated methods for correct inoculums, but advocate a minimal incubation time of 16 h and caution for visibly light inoculum, as strains could be reported falsely susceptible.
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Plateauing of prion infection in the spleen is consistent with earlier observations, and has been suggested to reflect an exhaustion of target cells (for review [22]) Importantly, the spleen tissue was highly infectious as it killed 100% of intracerebrally challenged mice within the minimal mean incubation time (∼500 days).
Using the ImageStreamX, we established the minimal number of exosomes and incubation time required to achieve cellular uptake.
Though time-dependent variations in PDMS surfaces can occur due to continuous surface degradation from the leaching of uncured low molecular weight oligomer siloxanes from the PDMS core into the surface, APTES remained stable on the surface over time in an aqueous environment, suggesting minimal effects from leaching during the incubation time.
While data are not available for Ebola, with most other viral infections, the greater the exposure inoculums (vomitus or blood touching the mucus membrane of health worker or family member during peak of illness) the shorter the incubation period, hence those with minor or minimal exposure may be more likely to show a longer incubation time.
In the study we used a minimal culture medium (containing 200 mg/L of peptone only), relatively long incubation time (14 days), and an initially very small bacterial concentration in the culture medium 10-100 CFU/mL).
As shown in Figure 1C, HHT did not induce cell death by itself, except for a minimal increase in A818-4 and Pt45P1 cells at 24 h (the longest incubation time).
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