Sentence examples for minimal ice from inspiring English sources

For optimal dehydration and minimal ice crystal formation, the medium contains both permeating and non-permeating cryoprotectants.

The yellow precipitate was filtered with a Buchner funnel, washed with minimal ice cold H2O, EtOH, and ether and then dried under vacuum.

The yellow precipitate was filtered off using a Buchner funnel, washed with minimal ice cold H2O, EtOH, and ether then dried under vacuum.

Optical properties indicate that the quantity of humic substances (HS) is minimal under an ice cover and DOM contains more microbial-derived peptide-like constituents.

The cell morphology indicated good cryopreservation with a minimal degree of ice crystal damage.

Cryopreserved PBMC (two vials from each donor) were transported to the participants on dry ice (minimal 5 kg/box), within 4 months after PBMC isolation and subsequent storage.

After rapid aspiration of PBS, a minimal volume of ice-cold 5% perchloric acid was added and the samples vortexed to ensure complete lysis.

Samples were lysed with Kontes pellet pestle for the minimal time required, incubated 30 min on ice and centrifuged 30 min at 4°C 17 900 rcf (13 000rpm).

Tissue samples kept in Earl's minimal essential medium on ice were trimmed to a suitable size, fixed in 4% paraformaldehyde and further processed for cryosectioning and immunostaining following the protocol used for embryos (see next subsection).

Blood samples were obtained without or with minimal haemostasis (<30 s), ice-cooled, and, immediately after clotting, centrifuged at 2000 g for 10 min.

They belonged to Bob Corey, 43, and Eric Grundman, 23, both ice fishermen with minimal interest in the Packers.