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The advent of real-time HSV PCR protocols now enables rapid result turnaround times with minimal hands on time.
Here we describe a protocol for automating the generation of these barcoded sequencing libraries, resulting in an increased robustness and minimal hands on time.
Once homozygous animals are obtained containing insertions, excision of the cassette can be achieved in 1 more week with minimal hands on time.
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Technically, both Q-PCR methods seem ideal for diagnostic applications (Table 5): minimal hands-on involvement, one-step, one piece of equipment, quick results, easy numeric analysis, easy troubleshooting.
This technology allows researchers to process hundreds of samples in one experiment with minimal hands-on time, thus considerably increasing gene expression profiling throughput.
DNA extraction can be done automated using the GenoXtract instrument requiring only minimal hands-on time.
This process was highly automated and required only minimal hands-on interaction by the operator.
The minimal hands-on three-day Infinium™ II protocol began with 750 ng of input DNA.
This assay provides rapid results with minimal hands-on time and low potential for PCR contamination [ 12– 12].
In Malaysia, the educational system is mostly didactic in nature, with very minimal hands-on, discussion and practical sessions.
The automation solution proposed here enables a high-throughput, cost-effective preparation of samples with minimal hands-on time.
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